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Positive CTC Detection Reagent 

Background Information

Positive CTC Selection Assay (EpCAM-magnetic beads) is designed to provide simple, sensitive and quantitative assessment of CTC enrichments by magnetically labeled CTC from peripheral blood. The EpCAM-magnetic beads use microscale particles with magnetic core, coupled with antibodies that recognize the EpCAM antigen. By using the magnetic field and the antibody specific binding ability, the magnetically labeled EpCAM cells could be temporarily immobilized on the column wall,  so the supernatant depleted of EpCAM cells can be discard easily and efficiently under magnetic attraction. Captured magnetically labeled EpCAM cells are visible and respond quickly to magnets, which are easy for manipulation.

Applications

Detection and enrichment of disseminated carcinoma cells in peripheral venous blood, for example, of patients with epithelial cancers, for subsequent analysis, e.g., flow, immunofluorescence, enumeration, cultivation, or RT-PCR.

Competitive Advantages

  • CTC capture rate  reaching  >95% (100 blood samples from patients with gastric, breast and colon cancers)

  • Captured CTC successful culture rate is 96%(out of 50 samples).

  • Our CTC Positive Selection Reagent has much higher sensitivity than other platforms and can capture cancer cells that other platforms can not (Can capture low EpCAM  expression cancer cell line, MDA-MB-231 )

Product Details

  • Product name:  Anti-EpCAM Monoclonal Antibody beads

  • Catalog number: BMI-EPCAMAb35-Beads

  • Size: 2ml

  • Gene: EpCAM

  • Purity: IgG purified

  • Isotype: IgG2b

  • Immunogen: Purified EpCAM

  • Localization: Cell membrane

  • Host: Mouse

  • Clone: EpAb3-5

  • Clonality: Monoclonal

  • Category: Primary

  • Target species: Human

  • Specificity:  This antibody has specificity for EpCAM

  • Species specificity: Cross-reacts with Human. Does not cross react with mouse

  • Dilution range: 20 reactions

  • Form:  Liquid (PBS (pH 7.4) with 0.09% NaN3)

  • Conjugate: protein G beads

  • Shipping and storage: 2-8°C, DO NOT FREEZE      

Product Information

References​

  1. Andree, Kiki C., et al. "Capture of tumor cells on anti-EpCAM-functionalized poly (acrylic acid)- coated surfaces." ACS applied materials & interfaces 8.23 (2016): 14349- 14356.

  2. Went, P.T., et al. “ Frequent EpCAM protein expression in human carcinomas.” Hum Pathol (2004): 35, 122-128. 

  3. Xu, H., et al.  “Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood.” Biomaterials (2011): 32, 9758-9765.

  4. Zborowski, M., and Chalmers, J.J. “Rare cell separation and analysis by magnetic sorting.” Anal Chem (2011): 83, 8050-8056.

  5. Imrich, S., Hachmeister, M., and Gires, O. “EpCAM and its potential role in tumor- initiating cells.” Cell Adh Migr(2012): 6, 30-38.

  6. Grover, P.K., et al. “Circulating tumour cells: the evolving concept and the inadequacy of their enrichment by EpCAM-based methodology for basic and clinical cancer research.” Ann Oncol (2014): 25, 1506-1516.

  7. Plouffe, B.D., Murthy, S.K., and Lewis, L.H. “ Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.” Rep Prog Phys. (2015): 78, 016601.

  8. Raimondi, C., Nicolazzo, C., and Gradilone, A.” Circulating tumor cells isolation:  the "post-EpCAM era". Chin J Cancer Res(2015): 27, 461-470.

  9. Schneck, H., Gierke, B., et al.” EpCAM-Independent Enrichment of Circulating Tumor Cells in etastatic Breast Cancer. “PLoS One (2015): 10, e0144535

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