BioMab

Background Information​

EpCAM (CD326), also known as tumor-associated calcium signal transducer 1 (TACSTD1) is a type I transmembrane glycoprotein of 39-42 kDa, consisting of 314 amino acids.  encoded by the TACSTD1 gene on the long arm of chromosome 2p21. It was originally identified as a dominant cell surface antigen on  human colon carcinoma. EpCAM is expressed on the basolateral membrane of all simple, pseudo-stratified, and transitional epithelia. It negatively regulates expression of p53 and p21, and promotes tumor cell growth, colony formation, migration and invasion. Anti-EpCAM monoclonal antibodies have shown promises as diagnostic tool and in antibody-based immunotherapies for a spectrum of malignancies. Comparative assay amongdifferent anti-EpCAM mAbs has shown that BioMAb’s BMI-EPCAMAb3 has the highest binding affinity, provides better sensitivity and specificity for downstream assays.

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Applications

WB, ELISA, FACS, IFA, IHC

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Product Details

• Product name:  Anti-EpCAM Monoclonal Antibody

• Catalog number: BMI-EPCAMAb35

• Size: 100ug

• Gene: EpCAM

• Purity: IgG purified

• Isotype: IgG2b

• Immunogen: Purified EpCAM

• Localization: Cell membrane

• Host: Mouse

• Clone: EpAb3-5

• Clonality: Monoclonal

• Category: Primary

• Target species: Human

• Specificity:  This antibody has specificity for EpCAM

• Species specificity: Cross-reacts with Human. Does not cross react with mouse

• Dilution range:  Flow Cytometry and ELISA:  0.01 to 1 μg/ml; WB, IHC and  IF:  0.1 to 1 μg/ml

• Form:  Liquid (PBS (pH 7.4) with 0.09% NaN3)

• Conjugate: unconjugated

• Shipping and storage: 2-8°C, DO NOT FREEZE

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