Anti-EpCAM Monoclonal Antibody
Background Information
EpCAM (CD326), also known as tumor-associated calcium signal transducer 1 (TACSTD1) is a type I transmembrane glycoprotein of 39-42 kDa, consisting of 314 amino acids. encoded by the TACSTD1 gene on the long arm of chromosome 2p21. It was originally identified as a dominant cell surface antigen on human colon carcinoma. EpCAM is expressed on the basolateral membrane of all simple, pseudo-stratified, and transitional epithelia. It negatively regulates expression of p53 and p21, and promotes tumor cell growth, colony formation, migration and invasion. Anti-EpCAM monoclonal antibodies have shown promises as diagnostic tool and in antibody-based immunotherapies for a spectrum of malignancies. Comparative assay amongdifferent anti-EpCAM mAbs has shown that BioMAb’s BMI-EPCAMAb3 has the highest binding affinity, provides better sensitivity and specificity for downstream assays.
Applications
WB, ELISA, FACS, IFA, IHC
Product Details
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Product name: Anti-EpCAM Monoclonal Antibody
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Catalog number: BMI-EPCAMAb35
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Size: 100ug
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Gene: EpCAM
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Purity: IgG purified
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Isotype: IgG2b
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Immunogen: Purified EpCAM
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Localization: Cell membrane
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Host: Mouse
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Clone: EpAb3-5
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Clonality: Monoclonal
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Category: Primary
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Target species: Human
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Specificity: This antibody has specificity for EpCAM
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Species specificity: Cross-reacts with Human. Does not cross react with mouse
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Dilution range: Flow Cytometry and ELISA: 0.01 to 1 μg/ml; WB, IHC and IF: 0.1 to 1 μg/ml
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Form: Liquid (PBS (pH 7.4) with 0.09% NaN3)
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Conjugate: unconjugated
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Shipping and storage: 2-8°C, DO NOT FREEZE
Product Information
References
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Andree, Kiki C., et al. "Capture of tumor cells on anti-EpCAM-functionalized poly (acrylic acid)-coated surfaces." ACS applied materials & interfaces 8.23 (2016): 14349-14356
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Liao, Mei-Ying, et al. Generation of an anti-EpCAM antibody and epigentic regulation of EpCAM in colorectal cancer International journal of Oncology 46:1788-1800 2015