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Anti-EpCAM Monoclonal Antibody 

Background Information

EpCAM (CD326), also known as tumor-associated calcium signal transducer 1 (TACSTD1) is a type I transmembrane glycoprotein of 39-42 kDa, consisting of 314 amino acids.  encoded by the TACSTD1 gene on the long arm of chromosome 2p21. It was originally identified as a dominant cell surface antigen on  human colon carcinoma. EpCAM is expressed on the basolateral membrane of all simple, pseudo-stratified, and transitional epithelia. It negatively regulates expression of p53 and p21, and promotes tumor cell growth, colony formation, migration and invasion. Anti-EpCAM monoclonal antibodies have shown promises as diagnostic tool and in antibody-based immunotherapies for a spectrum of malignancies. Comparative assay amongdifferent anti-EpCAM mAbs has shown that BioMAb’s BMI-EPCAMAb3 has the highest binding affinity, provides better sensitivity and specificity for downstream assays.

Applications

WB, ELISA, FACS, IFA, IHC

Product Details

  • Product name:  Anti-EpCAM Monoclonal Antibody

  • Catalog number: BMI-EPCAMAb35

  • Size: 100ug

  • Gene: EpCAM

  • Purity: IgG purified

  • Isotype: IgG2b

  • Immunogen: Purified EpCAM

  • Localization: Cell membrane

  • Host: Mouse

  • Clone: EpAb3-5

  • Clonality: Monoclonal

  • Category: Primary

  • Target species: Human

  • Specificity:  This antibody has specificity for EpCAM

  • Species specificity: Cross-reacts with Human. Does not cross react with mouse

  • Dilution range:  Flow Cytometry and ELISA:  0.01 to 1 μg/ml; WB, IHC and  IF:  0.1 to 1 μg/ml

  • Form:  Liquid (PBS (pH 7.4) with 0.09% NaN3)

  • Conjugate: unconjugated

  • Shipping and storage: 2-8°C, DO NOT FREEZE

WB

WB EpAB3-5.jpg

ELISA

ELISA EpAb3-5_edited.jpg

SPECIFICITY TEST

Specificity Test-EpAb3-5 PB.JPG
Specificity Test-EpAb3-5 MV.JPG

Product Information

References​

  1. Andree, Kiki C., et al. "Capture of tumor cells on anti-EpCAM-functionalized poly (acrylic acid)-coated surfaces." ACS applied materials & interfaces 8.23 (2016): 14349-14356

  2. Liao, Mei-Ying, et al. Generation of an anti-EpCAM antibody and epigentic regulation of EpCAM in colorectal cancer International journal of Oncology 46:1788-1800 2015

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