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Anti-CD45 Monoclonal Antibody

Background Information

CD45, also known as leukocyte common antigen (LCA), protein tyrosine phosphatase, receptor type I (PTPR) is a type I transmembrane protein encoded by PTPR gene specifically expresses in hematopoietic cells. PRPR gene has 34 exons. Dfferentially spliced transcription of exon 4, 5 and 6 yields 6 isoforms: ABC (exon 4, 5 and 6), AB (exon 4 and 5) or BC (Exon 5 and 6), A (only exon 4), B (only exon 5) and O (no exon 4, 5, or 6). Conventional monoclonal antibodies(mAbs) raised against CD45 have reactivity against all isoforms, while restricted mAB only reacts to specific isotypes (CD45RA, CD45RB, CD45RO). BMI-CD45Ab13, identifies all three major subsets of  leukocyte (Granulocyte, monocyte and lymphocyte) and was selected by Professor Leon Terstappen to participate in CANCER-ID Consortium.

Applications

This antibody is useful in Flow Cytometry, Enzyme-Linked Immuno Sorbent Assay, Western Blot, Immunocytochemistry, Immnofluorescence.

Product Details

  • Product name:  Anti-CD45 Monoclonal Antibody

  • Catalog number: BMI-CD45Ab13

  • Size: 100ug

  • Gene: CD45

  • Purity: IgG purified

  • Isotype: IgG2b

  • Immunogen: Purified CD45

  • Localization: Cell membrane

  • Host: Mouse

  • Clone: CD45-Ab-13

  • Clonality: Monoclonal

  • Category: Primary

  • Target species: Human

  • Specificity:  This antibody has specificity for CD45

  • Species specificity: Cross-reacts with Human. Does not cross react with mouse

  • Dilution range: Flow Cytometry, Enzyme-Linked ImmunoSorbent Assay 0.01~1 μg/ml,

  • Western Blot, Immunocytochemistry, Immnofluorescence 0.1~1 μg/ml

  • Form:  Liquid ( PBS (pH 7.4) with 0.09% NaN3)

  • Conjugate: unconjugated

  • Shipping and storage: 2-8°C, DO NOT FREEZE      

WB

CD45Ab-13 Western Blot.jpg

Flow Cytometry

CD45-Ab-13 Flow Cytometry.png

Specificity Test

CD45-Ab-13 Specificity Test.jpg

Immunostaining

CD45-Immunostaining -200.jpg

Product Information

 

​​References​

  1. Streuli M et al. (1987). Differential usage of three exons generates at least five different   mRNAs encoding human leukocyte common antigens. J. Exp. Med. 166, 1548–1566.

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