Anti-CD45 Monoclonal Antibody
Background Information
CD45, also known as leukocyte common antigen (LCA), protein tyrosine phosphatase, receptor type I (PTPR) is a type I transmembrane protein encoded by PTPR gene specifically expresses in hematopoietic cells. PRPR gene has 34 exons. Dfferentially spliced transcription of exon 4, 5 and 6 yields 6 isoforms: ABC (exon 4, 5 and 6), AB (exon 4 and 5) or BC (Exon 5 and 6), A (only exon 4), B (only exon 5) and O (no exon 4, 5, or 6). Conventional monoclonal antibodies(mAbs) raised against CD45 have reactivity against all isoforms, while restricted mAB only reacts to specific isotypes (CD45RA, CD45RB, CD45RO). BMI-CD45Ab13, identifies all three major subsets of leukocyte (Granulocyte, monocyte and lymphocyte) and was selected by Professor Leon Terstappen to participate in CANCER-ID Consortium.
Applications
This antibody is useful in Flow Cytometry, Enzyme-Linked Immuno Sorbent Assay, Western Blot, Immunocytochemistry, Immnofluorescence.
Product Details
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Product name: Anti-CD45 Monoclonal Antibody
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Catalog number: BMI-CD45Ab13
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Size: 100ug
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Gene: CD45
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Purity: IgG purified
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Isotype: IgG2b
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Immunogen: Purified CD45
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Localization: Cell membrane
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Host: Mouse
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Clone: CD45-Ab-13
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Clonality: Monoclonal
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Category: Primary
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Target species: Human
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Specificity: This antibody has specificity for CD45
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Species specificity: Cross-reacts with Human. Does not cross react with mouse
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Dilution range: Flow Cytometry, Enzyme-Linked ImmunoSorbent Assay 0.01~1 μg/ml,
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Western Blot, Immunocytochemistry, Immnofluorescence 0.1~1 μg/ml
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Form: Liquid ( PBS (pH 7.4) with 0.09% NaN3)
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Conjugate: unconjugated
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Shipping and storage: 2-8°C, DO NOT FREEZE
WB
Flow Cytometry
Specificity Test
Immunostaining
Product Information
References
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Streuli M et al. (1987). Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J. Exp. Med. 166, 1548–1566.